Fig. 4.

MiR-451a inhibits AKT/mTOR signaling pathway via YTHDC1-mediated PDPK1. (A-B) Rescue experiments were performed in HOS and 143B cells transfected with mimics NC, miR-451a mimics, miR-451a mimics + pcDNA3.1-YTHDC1, or miR-451a mimics + pcDNA3.1-YTHDC1-Mut. Q-PCR and western blot analyses of PDPK1 RNA and protein levels in the transfected HOS and 143B cells respectively. (C) Rescue experiments were performed in the indicated transfected HOS and 143B cells. Western blot analysis of p-AKT expression in the transfected HOS and 143B cells was shown. **P < 0.01.