Fig. 6.

MiR-451a hampers the tumorgenesis of osteosarcoma in vivo. (A) The volume of tumor xenografts in mice was detected every three days after seven days after treatment (i.e. injection with HOS or 143B cells expressing agomir NC or miR-451a agomir). (B) The weight of tumors excised from the mice sacrificed on the 28th day for xenografts. The weight of tumor xenografts was measured. (C-D) IHC analysis of cell proliferation markers (Ki67 and PCNA) in tumor xenografts in mice was shown. (E) TUNEL assay was performed to evaluate the apoptosis of paraffin-embedded tissues. (F-G) QPCR and western blot were applied to detect PDPK1 expression in mice tumor xenografts. (H) Western blot analysis of EMT markers in mice tumor xenografts. **P < 0.01.