TABLE 1.
Resistance patterns of RMP-resistant M. tuberculosis isolates from Hungary by the proportion method, the line probe assay, and DNA sequencing of the 81-bp region of the rpoB genea
| Strain | Result by method:
|
Mutation
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Proportion method
|
LiPA
|
|||||||||||||||
| SM | INH | RMP | EMB | TB-P | S1 | S2 | S3 | S4 | S5 | R2 | R4a | R4b | R5 | LiPA | Seq. | |
| 1 | S | R | R | R | + | + | − | + | + | + | + | − | − | − | D516V | D516V |
| 2 | S | R | R | S | + | + | + | + | + | − | − | − | − | + | S531L | S531L |
| 3 | S | R | R | R | + | + | − | + | + | + | + | − | − | − | D516V | D516V |
| 4 | S | R | R | S | + | + | + | + | + | − | − | − | − | + | S531L | S531L |
| 5 | R | S | R | S | + | + | + | + | + | − | − | − | − | + | S531L | S531L |
| 6 | S | S | R | S | + | + | + | − | − | +/− | − | − | − | − | UDR | Deletion 522–525 |
| 7 | S | R | R | R | + | + | − | + | + | + | + | − | − | − | D516V | D516V |
| 8 | S | R | R | R | + | + | − | + | + | + | + | − | − | − | D516V | D516V |
| 9 | R | R | R | S | + | + | + | + | − | + | − | − | + | − | H526D | H526D |
| 10 | S | R | R | R | + | + | − | + | + | + | + | − | − | − | D516V | D516V |
| 11 | R | R | R | R | + | + | − | + | + | + | + | − | − | − | D516V | D516V |
| 12 | R | R | R | R | + | + | − | + | + | + | + | − | − | − | D516V | D516V |
| 13 | S | R | R | R | + | + | + | + | + | − | − | − | − | + | S531L | S531L |
| 14 | R | R | R | R | + | + | + | + | + | − | − | − | − | + | S531L | S531L |
| 15 | S | R | R | R | + | + | − | + | + | + | + | − | − | − | D516V | D516V |
| 16 | S | R | R | R | + | + | − | + | + | + | + | − | − | − | D516V | S509T/D516V |
| 17 | S | R | R | S | + | − | + | + | + | + | − | − | − | − | UDR | Q513K |
| 18 | S | R | R | R | + | + | + | + | + | + | − | − | − | − | UD | WT |
| 19 | S | R | R | R | + | + | + | + | + | − | − | − | − | + | S531L | S531L |
| 20 | R | R | R | S | + | +/− | + | + | + | + | − | − | − | − | UDR | Q513P |
| 21 | S | R | R | S | + | + | + | + | + | − | − | − | − | + | S531L | S531L |
| 22 | S | R | R | S | + | + | + | + | − | + | − | − | + | − | H526D | H526D |
| 23 | S | R | R | R | + | + | − | + | + | + | + | − | − | − | D516V | D516V |
| 24 | S | R | R | R | + | + | − | + | + | + | + | − | − | − | D516V | D516V |
| 25 | S | R | R | S | + | + | − | + | + | + | + | − | − | − | D516V | D516V |
| 26 | S | S | R | S | + | + | + | + | + | − | − | − | − | + | S531L | S531L |
| 27 | R | R | R | R | + | + | + | + | + | − | − | − | − | + | S531L | S531L |
| 28 | R | R | R | R | + | + | + | + | + | + | − | − | − | − | WT | WTb |
| 29 | R | R | R | R | + | + | + | + | + | + | − | − | − | − | WT | WT |
| Co. 1 | S | S | S | S | + | + | + | + | − | − | − | − | − | − | UDR | R529R |
| Co. 2 | S | S | S | S | + | + | + | + | + | + | − | − | − | − | WT | WT |
| Co. 3 | S | S | S | S | + | + | + | + | + | + | − | − | − | − | WT | WT |
| Co. 4 | S | S | S | S | + | + | + | + | + | + | − | − | − | − | WT | WT |
| Co. 5 | S | S | S | S | + | + | + | + | + | + | − | − | − | − | WT | WT |
| Co. 6 | S | S | S | S | + | + | + | + | + | + | − | − | − | − | WT | WT |
| H37Rv | S | S | S | S | + | + | + | + | + | + | − | − | − | − | WT | WT |
a
Abbreviations: Co., control; Seq., DNA sequencing of the hypervariable region; S, susceptible; R, resistant; +, positive; −, negative; +/−, indeterminate; UD, undetermined; UDR, undetermined resistant; WT, wild type. Probes R2, R4a, R4b, and R5 are specific for mutations D516V, H526Y, H526D, and S531L, respectively.
b
A V146F mutation was found in this isolate.