TABLE 2.
C t value and clinical performance comparison of each of the target genes for real‐time reverse‐transcription PCR assays
| Genes | Allplex SARS‐CoV‐2/FluA/FluB/RSV kit | Standard M nCoV real‐time detection kit | U‐TOP COVID‐19 detection kit | ||||||
|---|---|---|---|---|---|---|---|---|---|
| S | RdRp | N | RdRp | E | ORF1ab | N | S | E | |
| C t value (mean ± SD) | 26.44 ± 6.94 | 26.52 ± 7.29 | 25.28 ± 6.72 | 25.76 ± 6.89 | 25.65 ± 6.71 | 27.62 ± 6.66 | 28.20 ± 6.49 | 28.34 ± 6.69 | 27.72 ± 6.53 |
| Sensitivity (95% CI) | 98.88 (93.90–99.97) | 97.75 (92.12–99.73) | 95.51 (88.89–98.76) | 100 (95.94–100) | 97.75 (92.12–99.73) | 97.75 (92.12–99.73) | 96.63 (90.46–99.30) | 94.38 (87.38–98.15) | 97.75 (92.12–99.73) |
| Specificity (95% CI) | 99.38 (96.57–99.98) | 100 (97.72–100) | 100 (97.72–100) | 100 (97.72–100) | 100 (97.72–100) | 100 (97.72–100) | 100 (97.72–100) | 100 (97.72–100) | 100 (97.72–100) |
The clinical performance was evaluated based on the reference standard results, which was defined by the consensus criterion.
Abbreviations: CI, confidence interval; C t , cycle threshold; SD, standard deviation