| Reagent/Resource | Reference or Source | Identifier or catalogue number |
|---|---|---|
| Experimental models | ||
| HEK‐293 Flp‐In cells (H. sapiens) | ThermoFisher | R75007 |
| HEK‐293T cells (H. sapiens) | ATCC | CRL‐3216 |
| Electrocompetent E. cloni cells (10G ELITE) | Lucigen | 60052‐4 |
| CFBE cells (H. sapiens) |
Molinski et al (2015) Botelho et al (2015) |
N/A |
| Recombinant DNA | ||
| MaMTH reporter vector using the pcDNA3.1(−) backbone | Gibson et al (2009) and Saraon et al (2020) | N/A |
| MaMTH bait destination vector | Gateway cloning technology (Thermo Fisher) and Saraon et al (2020) | N/A |
| wt‐ and F508del‐CFTR cDNA entry constructs | Dr. Johanna Rommens lab | Ref Seq: NM_000492.3 |
| MaMTH prey destination vector | Saraon et al (2020) | N/A |
| Plasmid library generation | Human ORFeome V8.1 collection (Yang et al, 2011) | N/A |
| Antibodies | ||
| Anti‐Flag antibody | Sigma | F‐1804 |
| Anti‐mouse IgG antibody conjugated with Alexa 647 | Invitrogen | A‐31571 |
| Anti‐CFTR 596 | Cystic Fibrosis Foundation | N/A |
| Anti‐calnexin | BD Transduction Laboratories | 610523 |
| HRP‐conjugated goat anti‐mouse IgG | Bio‐Rad | 170‐6516 |
| Anti‐FLAG antibody | Millipore Sigma | F3165 |
| Anti‐V5 antibody | Cell Signaling Technology | 13202S |
| Oligonucleotides and other sequence‐based reagents | ||
| PCR primers | This study | Table EV1 |
| Custom library of 421 Ambion Silencer Select siRNAs | This study | Table EV3 |
| Non‐targeting Neg1 negative control siRNA | Ambion | 4390844 |
| Chemicals, enzymes and other reagents | ||
| DMEM | Wisent | 319‐005‐CL |
| DMEM high glucose | Corning | 10‐013‐CV |
| Opti‐MEM | Gibco | 31985070 |
| IntestiCultTM Human OGM | STEMCELL Technologies | 06010 |
| FBS | Gibco | 10270 |
| Hygromycin | Bioshop | HYG003.10 |
| Blasticidin | Invivogen | ant‐bl |
| Puromycin | Invivogen | ant‐pr |
| Doxycycline | Sigma | 9891 |
| Primocin | Invivogen | ant‐pm |
| Vancomycin | Sigma | SBR00001 |
| Gentamycin | Sigma | G1272 |
| Y‐2632 (ROCK pathway inhibitor) | Sigma | SCM075 |
| Forskolin | Sigma Aldrich | F3917 |
| VX‐661 | Selleckchem | S7059 |
| VX‐809 | Selleckchem | S1565 |
| VX‐445 | Selleckchem | S8851 |
| CFTRinh‐172 |
Cystic Fibrosis Foundation EMD Millipore Corp |
N/A |
| Amiloride | Spectrum Chemical | TCI‐A2599 |
| Phire Tissue Direct Dilution Buffer | Thermo Scientific | N/A |
| Phire Tissue Direct PCR Master Mix | Thermo Scientific | N/A |
| QIAquick PCR Purification Kit | Qiagen | N/A |
| Qiagen Mini Kit | Qiagen | 27106 |
| Qiagen Micro Kit | Qiagen | 74004 |
| iSCRIPT cDNA synthesis kit | Bio‐Rad | 1708890 |
| SsofastTM EvaGreen fluorophore | Bio‐Rad | 172‐5200 |
| CellMask | Invitrogen | H32712 |
| ER‐Tracker | Invitrogen | E34250 |
| Hoechst 33342 | Sigma | B2261 |
| Calcein green | Invitrogen | C34852 |
| X‐tremeGENE 9 DNA Transfection Reagent | Roche | 6365787001 |
| Lipofectamine 2000 | Invitrogen | 11668019 |
| Lipofectamine LTX | Thermo Fisher Scientific | 15338100 |
| Laemmli sample buffer | Bio‐Rad | 161‐0747 |
| Protease inhibitor cocktail | Roche | 11873580001 |
| Clarity Western ECL Substrate | Bio‐Rad | 1705061 |
| Blue membrane potential dye | Molecular devices | R8034 |
| 384 well plates | Falcon | 736‐2044 |
| 96 well plates | Sarstedt | 83.3924 |
| 12 well plates | SPL Life Sciences | 30012 |
| PureCol®‐coated Transwell | Corning | CostarTM 3470 |
| Non‐perfused Ussing chamber | Physiologic Instruments | N/A |
| Drying pearls | Sigma‐Aldrich | 94098 |
| μMACSTM FLAG magnetic microbeads and columns | Miltenyi Biotec | N/A |
| Matrigel | BD Biosciences | N/A |
| Software | ||
| GraphPad Prism | https://www.graphpad.com | N/A |
| Integrated Interactions Database (IID) |
PMID: 34755877 |
Version 2021‐05 |
| NaViGaTOR | http://navigator/ophid.utoronto.ca/navigatorwp | Version 3.0.15 |
| R programming language/Bowtie 2 Alignment Tool | R Core Team, Bowtie2 alignment tool (Langmead & Salzberg, 2012) | N/A |
| CellProfiler | McQuin et al (2018) | N/A |
| Image Lab | Bio‐Rad | N/A |
| MATLAB‐based organoid swelling capturing program | MATLAB | N/A |
| Other | ||
| NexteraXT library preparation kit | Illumina | N/A |
| NextSeq500 System | Illumina | N/A |
| HiSeq2500 System | Illumina | N/A |
| BD FACS Melody | BD Biosciences | N/A |
| Rank product method | Breitling et al (2004) | N/A |
| Genevac miVac Duo | Thermo Fisher Scientific | N/A |
| MultidropTM Combi peristaltic dispenser | Thermo Scientific | 5840300 |
| Leica DMI 6000B | Leica | N/A |
| ChemiDoc XRS | Bio‐Rad | N/A |
| Cellomics ArrayScan, VTI HCS Reader | Thermo Fisher Scientific | N/A |
| NanoDrop 2000 | Thermo Fisher Scientific | N/A |