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. 2016 Nov 28;10:BCBCR.S40856. doi: 10.4137/BCBCR.S40856

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© 2016 SAGE Publications.

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Flow cytometric detection of circulating tumor cells (CTCs) and circulating CSCs. (A) CD45 and side scatter histogram were used to select the CD45^– cells (R1). (B) The expression of cytokeratin in CD45^– cells (R1) was detected. CTCs were defined as cytokeratin⁺CD45^– (R2). (C) The CTCs were further gated to detect CSCs, which are identified as cytokeratin⁺CD44⁺CD24^– CD45^– cells.