Figure 6.

Level of transcripts coding for proteins involved in immune responses after the CyHV-3 challenge at 2 dpc and 7 dpc measured with classical RT-qPCR and integrated fluidic circuit nanoscale RT-qPCR in skin. Presented are selected markers for T cell response: (A) cd4, (B) cd8b1, (C) tcra2, B cell response: (D) igm, (E) igz2, (F) grp183 which is also involved in oxysterol response, antiviral response (G) mx2, neutrophil-specific response: (H) mpo, oxysterol response: (I) ch25h_b, pro-inflammatory response: (J) il1b, anti-inflammatory response: (K) il10, stress response: (L) hsp70.The description of experimental groups: CB – BSA, non-vaccinated, unchallenged control, CD – defensins, non-vaccinated, unchallenged control, CVB – BSA, vaccinated, unchallenged control, CVD – defensins, vaccinated, unchallenged control, KB - BSA non-vaccinated, CyHV-3 challenged, KD – defensins non-vaccinated, CyHV-3 challenged, KVB – BSA, vaccinated, CyHV-3 challenged, KVD – defensins, vaccinated, CyHV-3 challenged. The results are presented as 25%-75% box plots with min. and max. values as whiskers with indication of all data points. The data are shown as normalised copy numbers. * indicates statistical significant difference at p < 0.05. Statistical analysis was performed with two-way ANOVA with multiple comparisons test performed with the Holm-Sidak method.