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. 2022 Jan 31;13:787021. doi: 10.3389/fimmu.2022.787021

Figure 8.

Figure 8

Bacterial load and level of 16S rRNA for selected bacteria species after challenge with CyHV-3 measured with qPCR or RT-qPCR. (A) All bacteria (unbact), flavobacteria (flavo), aeromonads (aero), pseudomonads (pseud) and streptococci (strep) load in skin at 7 dpc. Expression of bacterial 16S rRNA: (B) all bacteria 16S in skin at 2 dpc and 7 dpc, (C) flavobacteria 16S in skin at 2 dpc and 7 dpc, (D) aeromonas 16S in skin at 2 dpc and 7 dpc. The description of experimental groups: CB – BSA, non-vaccinated, unchallenged control, CD – defensins, non-vaccinated, unchallenged control, CVB – BSA, vaccinated, unchallenged control, CVD – defensins, vaccinated, unchallenged control, KB - BSA non-vaccinated, CyHV-3 challenged, KD – defensins non-vaccinated, CyHV-3 challenged, KVB – BSA, vaccinated, CyHV-3 challenged, KVD – defensins, vaccinated, CyHV-3 challenged. The results are presented as 25%-75% box plots with min. and max. values as whiskers with indication of all data points. Bacteria load data are shown as genomic 16S copy numbers normalised for 250 ng of extracted DNA. The expression data are shown as normalised copy numbers. * indicates statistical significant difference at p < 0.05. Statistical analysis was performed with two-way ANOVA with multiple comparisons test performed with the Holm-Sidak method.