Figure 2.

Cytokine induction in response to mono-infection of P. aeruginosa or S. mitis or co-infection of them in mouse PCLS. (A) A brief scheme of PCLS generation workflow. (B) PCLS were non-infected (Ctrl) or mono-infected with P. aeruginosa PAO1 (Pae), S. mitis SM4 (Smi) or co-infected with both of them (Co-infec). Release of several cytokines into the supernatant was assessed after 4 h of infection followed by overnight incubation. Data are presented as Mean ± SEM of three independent experiments. * indicate significant difference between co-infection and P. aeruginosa mono-infection. **p < 0.01, ***p < 0.001, ****p < 0.0001.