TABLE 2.
Description of the antibodies used to define ILCs.
| Marker | Label | Source | Clone | Dilution | |
|---|---|---|---|---|---|
| Lineage markers | CD3 | FITC | BD | UCHT1 | 1/40 |
| CD19 | FITC | BD | HIB19 | 1/40 | |
| CD14 | FITC | BD | M5E2 | 1/100 | |
| CD11b | FITC | BD | M1/70 | 1/100 | |
| CD11c | FITC | BD | B-ly6 | 1/100 | |
| CD34 | FITC | BD | 581 | 1/40 | |
| CD56 | FITC | BD | B519 | 1/100 | |
| ILC surface markers | CD45 | APC-Cy7 | BD | 2D1 | 1/200 |
| CD127 (IL7Ra) | PerCP-Cy5.5 | BD | HIL-7R-M21 | 1/50 | |
| CRTH2 (CD294) | PE-Cy7 | BioLegend | BM16 | 1/50 | |
| CD117 (cKit) | PE | BD | YB5.B8 | 1/50 | |
| NKp44 (CD336) | APC | BD | p44-8 | 1/20 | |
| ILC intracellular markers | T-bet (TBX21) | PE-CF594 | BD | O4-46 | 1/20 |
| GATA3 | BV421 | BD | L50-823 | 1/20 | |
| RORγt | BV650 | BD | Q21-559 | 1/20 | |
| IFN-γ | BV605 | BD | B27 | 1/20 | |
| IL-4 | BV786 | BD | MP4-25D2 | 1/20 | |
| IL-17A | BV510 | BD | N49-653 | 1/20 |
Note. ILCs, innate lymphoid cells; FITC, fluorescein isothiocyanate.