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. 2022 Jan 31;12:786543. doi: 10.3389/fcimb.2022.786543

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Copyright © 2022 Jiang, Wang, Zhu, Hu, Lin and Pan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Presence of Sja-miR-71a in the infected hepatocytes. (A) PCR analyses of parasite NADH gene excludes contamination with parasite RNA. Lane 1: sample of parasite positive control; Lanes 2 and 3: samples of hepatocyte of mice infected with S. japonicum (infected); Lanes 4 and 5: samples of hepatocytes of normal mice (uninfected); (B) qPCR analyses of sja-miR-71a in the hepatocytes of mice during the parasite infection. (C) The PAGE electrophoresis of PCR products from the samples of S. japonicum infected (Lanes 2 and 3) or normal mice (Lanes 4 and 5) hepatocytes. Sja-miR-71a mimic were used as positive control (Lane 1). (D) The sequence alignment of reference sja-miR-71a and PCR products of infected hepatocytes.