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. 2022 Feb 14;20:88. doi: 10.1186/s12967-022-03282-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Verification of decreased HPN expression in HCC and correlation with expression of marker genes for immune cells. a Western blot analysis of HPN in another cohort of human samples. General picture (b), representative images of H&E staining (c) and western blot analysis of HPN (d) in a BALB/c mouse model. e Representative images of immunohistochemical staining for CD68, CD163 and IL-10 in a BALB/c mouse model (Scale bar, 100 μm). Data are expressed as mean ± SEM. Statistical analysis was performed according to a Mann–Whitney test. Normal means healthy subjects based on human liver samples. Control means control group based on the HCC mouse model. H&E, haematoxylin and eosin. **P < 0.01, ***P < 0.001 vs Normal or Control. HCC hepatocellular carcinoma. (n = 6 for each group)