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. Author manuscript; available in PMC: 2022 Feb 14.
Published in final edited form as: Oncogene. 2021 Feb 9;40(10):1806–1820. doi: 10.1038/s41388-021-01667-y

Figure 4. ACSL1 regulates the biosynthesis of acyl-CoAs in prostate cancer cells.

Figure 4.

(A-T) The amount of C16:0, C18:0, C18:1, and C18:2-CoA. 22Rv1, PC-3, DU145, PNT2, and 293T cells were transduced with shRNA-control (white bars) or shRNA-ACSL1 (black bars) by lentiviral infection. The transduced cells were grown in the medium with/without palmitic acid (PA) (400 μM). The levels of C16:0-CoA (A-E), C18:0-CoA (F-J), C18:1-CoA (K-O), and C18:2-CoA (P-T) were measured by LC-MS/MS. The amount of C12:0- and C14:0-CoAs are presented in Supplementary Figure 6. Data are represented as mean ± SD (n=3). Student’s t test; #, *: p<0.05; ##, **: p<0.01.