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. Author manuscript; available in PMC: 2022 Feb 14.
Published in final edited form as: Oncogene. 2021 Feb 9;40(10):1806–1820. doi: 10.1038/s41388-021-01667-y

Figure 5. Knockdown of ACSL1 inhibits lipid accumulation in prostate cancer cells.

Figure 5.

22Rv1, PC-3, and DU145 cells transduced with shRNA-control or shRNA-ACSL1 were grown in the ATCC recommended medium with a mixture of oleic acid (OA, 400 μM) and palmitic acid (PA, 200 μM) for 48 h. (A-F) Total triglyceride (TG) and phospholipid (PL) (A-C) and acyl chain components of total triglyceride (D-F) were analyzed by gas chromatography. Data are represented as mean ± SD (n=3). One-way ANOVA and Tukey post hoc test; *: p<0.05; **: p<0.01. (G-I) The accumulation of cellular lipids was visualized by Oil Red O staining in 22Rv1 (G), PC-3 (H), and DU145 cells (I). A representative image of each group is shown.