A) Weight change (%), B) Gonadal fat pad mass (g), C) H&E stained adipose tissue sections, D) average adipocyte diameter (μm), and E) average daily food consumption (g/mouse/day) in C57BL/6J mice fed a high-fat, high-sugar diet for 8 weeks (HFHS) then either a low-fat diet (LFD) or low-fat diet supplemented with TM5441 (LFD+TM) for an additional 2, 4, or 8 weeks (i.e. week 10, 12, or 16, respectively.) Values are expressed as mean (n=6) ± SD, * p<0.05. Additionally, there was a statistically significant difference (p<0.05) in gonadal fat pad mass and adipocyte diameter (panels B,D) among mice fed a HFHS for 8 weeks compared to all other cohorts.