A) Representative H&E stained liver section, B) hepatic triglyceride content (mg trig per gram liver) in C57BL/6J mice fed a high-fat, high-sugar diet for 8 weeks (HFHS) then either a low-fat diet (LFD) or low-fat diet supplemented with TM5441 (LFD+TM) for an additional 2, 4, or 8 weeks (i.e. week 10, 12, or 16, respectively), C) hepatic mRNA expression of fatty acid oxidation genes, D) Plasma ALT (IU/L) and E) hepatic mRNA expression of pro-inflammatory genes in C57BL/6J mice fed a HFHS diet for 8 weeks then either LFD or LFD+TM for an additional 2 weeks. Values are expressed as mean (n=6) ± SD, * p<0.05.