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. Author manuscript; available in PMC: 2022 Feb 14.
Published in final edited form as: Cell Rep. 2022 Feb 1;38(5):110312. doi: 10.1016/j.celrep.2022.110312

Figure 6. Zic5 interacts with Gli3.

Figure 6.

(A) GFP or GFP-Zic5 DNA was transfected in HEK293T cells. Co-IP using GFP antibody shows exogenous Zic5 interacts with endogenous Gli3 but not Gli2.

(B) Zic5 interacts with Gli3 endogenously in HEK293T cell

(C) Illustration of the 3rd-5th ZF domains deletion mutants for Zic5 and Gli3.

(D and E) Zic5 interacts with Gli3 through the 3rd-5th ZF domains.

(F) Co-IPs using HA antibody on lysates from chopped embryo heads injected with indicated RNA shows that Zic5 interacts with endogenous Gli3 through the 3rd-5th ZF domains.

(G) Cell immunofluorescence assays in HEK293T cells stably expressing Gli3-flag with indicated plasmids transfected. Histograms represent the percentage of cells with indicated Gli3-flag subcellular localization. scale bar, 10 μm.

(H) Co-IPs using the indicated antibody on lysates of HEK293T cells transfected with indicated plasmids shows that Zic5-GR interacts with Gli3.