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. 2022 Feb 14;11:e72981. doi: 10.7554/eLife.72981

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Strain, strain background (Mus musculus) C57BL/6 J mice Orient Bio N/A
Strain, strain background (Mus musculus) ICR mice; CrljOri:CD1 Orient Bio N/A
Genetic reagent (Mus musculus) Gad2-IRES-Cre mice; Gad2tm2(cre)Zjh/J Jackson Laboratories Stock No: 010802; RRID:IMSR_JAX:010802
Genetic reagent (Mus musculus) Pcp2-cre mice; B6.129-Tg(Pcp2-cre)2Mppin/J Jackson Laboratories Stock No: 004146; RRID:IMSR_JAX:004146
Genetic reagent (Mus musculus) Ai six reporter mice; B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J Jackson Laboratories Stock No: 007906; RRID:IMSR_JAX:007906
Antibody Mouse monoclonal anti-calbindin Sigma-Aldrich Cat#C9848; RRID:AB_476894 (1:200)
Antibody Rabbit polyclonal anti-TH Millipore Cat#AB152; RRID: AB_390204 (1:400)
Antibody Rabbit monoclonal anti-c-Fos Cell Signalling Technology Cat#2250; RRID: AB_2247211 (1:300)
Antibody Mouse monoclonal anti-NeuN Millipore Cat#MAB377; RRID: AB_2298772 (1:200)
Antibody Mouse monoclonal anti-GAD67 Millipore Cat#MAB5406; RRID:AB_2278725 (1:1000)
Antibody Mouse monoclonal anti-GFAP Millipore Cat#MAB360; RRID:11212597 (1:200)
Antibody Goat anti-mouse IgG(H + L) alexa fluor@647 Invitrogen Cat#A21235; RRID: AB_2535804 (1:200)
Antibody Goat anti-rabbit IgG(H + L) alexa fluor@647 Invitrogen Cat#A21245; RRID: AB_141775 (1:200)
Antibody Rabbit polyclonal anti-OLLAS Antibodies-online ABIN1842163 (1:1000)
Chemical compound, drug Clozapine N-oxide Hello Bio Cat#HB1807 10 mg/kg, i.p.
Chemical compound, drug Bicuculline
methochloride
Tocris Bioscience Cat#0131
Chemical compound, drug Mounting medium Vector Laboratories Cat#H-1400
Chemical compound, drug Mounting medium
with DAPI
Vector Laboratories Cat#H-1500
Chemical compound, drug Prolong diamond
antifade mountant
Thermo Fisher Scientific Cat#P36961
Chemical compound, drug Cholera toxin
subunit B, Alexa
flour 647 conjugate
Thermo Fisher Scientific Cat#C34778
Chemical compound, drug Tetrodotoxin Tocris Bioscience Cat#1,069
Chemical compound, drug 4-Aminopyridine Tocris Bioscience Cat#0940
Recombinant DNA reagent pAAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Addgene plasmid #44,361
Recombinant DNA reagent pAAV-hSyn-DIO-hM4Di(Gi)-mCherry Addgene Addgene plasmid #44,362
Recombinant DNA reagent pAAV-CaMKIIa-hChR2(H134R)-EYFP Addgene Addgene plasmid #26,969
Recombinant DNA reagent AAV-CaMKIIa-
GCaMP6f-P2A-nls-dTomato
Addgene Addgene plasmid #51,087
Recombinant DNA reagent pBAD-mTagBFP2 Addgene Addgene plasmid #34,632
Recombinant DNA reagent ptdTomato-N1 Clontech Cat#632,532
Recombinant DNA reagent paavCAG-iCre Addgene Addgene plasmid #51,904
Recombinant DNA reagent paavCAG-sfGFP Addgene Will be deposited
Recombinant DNA reagent paavCAG-pre-
mGRASPi-
mCerulean
Addgene Will be deposited
Recombinant DNA reagent paavCAG-post-mGRASPi-2AdT Addgene Addgene plasmid #34,912
Recombinant DNA reagent rAAV2-retro helper Addgene Addgene plasmid #81,070
Biological sample (AAV) AAV-sSyn-GFP Kim et al., Kim et al., 2015a
Biological sample (AAV) AAV-sSyn-tdT This paper Further information will be provided by the Lead Contact upon request.
Biological sample (AAV) AAV-sSyn-mTBFP This paper Further information will be provided by the Lead Contact upon request.
Biological sample (AAV) AAV-sSyn-ChR2-GFP This paper Further information will be provided by the Lead Contact upon request.
Biological sample (AAV) AAV-sSyn-ChR2-2AdT This paper Further information will be provided by the Lead Contact upon request.
Biological sample (AAV) AAV-sSyn-FLEX-GFP This paper Further information will be provided by the Lead Contact upon request.
Biological sample (AAV) AAV-sSyn-FLEX-ChR2-GFP This paper Further information will be provided by the Lead Contact upon request.
Biological sample (AAV) AAV-sSyn-FLEX-hM3Dq(Gq)–2AGFP This paper Further information will be provided by the Lead Contact upon request.
Biological sample (AAV) AAV-sSyn-FLEX-hM4Di(Gi)–2AGFP This paper Further information will be provided by the Lead Contact upon request.
Software, algorithm pClamp 10 Axon instruments RRID:SCR_011323
Software, algorithm NIS-Element Nikon RRID:SCR_014329
Software, algorithm Fiji NIH RRID:SCR_002285
Software, algorithm Ethovision Noldus RRID:SCR_000441
Software, algorithm Origin Origin Lab RRID:SCR_014212