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. 2022 Feb 7;27(1):32–44. doi: 10.1080/13510002.2022.2036507

Figure 2.

Figure 2.

LBP alleviates PM2.5-induced oxidative stress and oxidative damage in HaCaT cells. (A) Cells were double-stained with Hochest33342 and DCFH-DA, and intracellular ROS levels were observed by a fluorescent microscopy. Scale bar = 100 μm. (B) Statistics of intracellular ROS level, stained with DCFH-DA. The intracellular MDA level (C) and SOD activity (D) were detected by the corresponding assay kits. (E) Cells were double-stained with Hochest33342 and DHE, and intracellular ROS levels were observed by a fluorescent microscopy. Scale bar = 100 μm. Values are mean ± SD. *p < 0.05, **p < 0. 01, ***p < 0.001 versus the control group; #p < 0.05, ##p < 0.01, ###p < 0.001 versus the PM2.5 treatment alone group.