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. 2022 Feb 15;132(4):e152297. doi: 10.1172/JCI152297

Figure 3. TAB2-deficient mice are predisposed to adverse cardiac remodeling and dysfunction after pathological stress.

Figure 3

(A and B) Western blot and quantification of cardiac TAB2 levels normalized to GAPDH in WT mice subjected to TAC versus sham (A) or MI versus sham (B) for 2 weeks. *P < 0.05 versus sham. n = 4. (C) Western blot and quantification of cardiac TAB2 levels in Tab2fl/fl and Tab2fl/fl-αMHC-Cre mice at 2 months of age. *P < 0.05 versus Tab2fl/fl. n = 3. (D) Masson’s trichrome–stained cardiac sections from mice of the indicated genotypes 1 week after TAC. (E) Quantification of cardiac fibrosis. *P < 0.05 versus sham. #P < 0.05 versus Tab2fl/fl TAC. n = 6–7. (F and G) Echocardiographic measurement of FS and LVED. *P < 0.05 versus sham. #P < 0.05 versus Tab2fl/fl TAC. (H) HW/BW ratio of the indicated mice. *P < 0.05 versus sham. (I) LW/BW ratio of the indicated mice. *P < 0.05 versus sham. #P< 0.05 versus Tab2fl/fl TAC. (J) Masson’s trichrome–stained cardiac sections from the indicated mice 1 week after MI. (K) Infarct scar size of the indicated mice subjected to MI for 1 week (left). Initial area at risk (AAR) normalized to area of the left ventricle (LV) was measured 24 hours after MI. *P < 0.05 versus Tab2fl/fl. n = 6. (L and M) FS and LVED measured by echocardiography. *P < 0.05 versus sham. #P < 0.05 versus Tab2fl/fl MI. n = 5–6. (N and O) HW/BW and LW/BW ratios. *P < 0.05 versus sham. #P < 0.05 versus Tab2fl/fl MI. n = 5–6. Data were analyzed by Student’s t test for comparisons between 2 groups and by 2-way ANOVA with Tukey’s multiple-comparison test for consideration of genotypes and treatments.