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. 2022 Feb 9;17:603–616. doi: 10.2147/IJN.S347506

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© 2022 Wei et al.

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Synergistic effect of HA-PS@NPs to reduce LPS-induced macrophage inflammation. (A) ROS levels with different treatments were evaluated. ROS probe, DCFH-DA (10μM), was added to the cells and incubated for 30 min. Rosup (0.5mg/mL) treated group was a positive control. Fluorescence of cells was observed by fluorescence microscope with 488 nm excitation and 525nm emission. (B) The fluorescence intensity of each group was analyzed by Image J software, (n=6), **p<0.01. (C–E) mRNAs expression of TNF-α, IL-1β and IL-6 in Raw 264.7 cells with different treatments were evaluated by real-time PCR, (n=6), **p<0.01, ***p<0.01.