TREM2 overexpression suppresses NF-κB activation and neuroinflammation in the hippocampus. (A, B) Western blot membranes depicting representative band intensities for p65, p-p65, IkB-α, and p-IkB-α; normalize to β-actin and α-tubulin; n = 6 per group. (C) Relative mRNA levels of the M1 markers and proinflammatory factors iNOS, TNF-α, IL-1β, TLR-4 and IL-6 and the M2 markers Arg-1 and YM1/2; n = 5 per group, asterisks (∗) vs HFD-con, asterisks (#) vs NCD-con. The data represent the mean ± SEM, ∗/#P < 0.05, ∗∗/##P < 0.01, ∗∗∗P < 0.001 (one-way ANOVA with Tukey's post hoc test).