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. 2020 Aug 10;9(2):456–465. doi: 10.1016/j.gendis.2020.07.013

Figure 2.

Figure 2

lnc-HCAR modulates Vegfa and Mmp13 in hypertrophic chondrocytes during the chondrocytes hypertrophy without regulate matrix mineralization in vitro.(A) Schematic diagram of the model for hypertrophic differentiation in vitro. (B) Quantitative analysis of the expression level of lnc-HCAR by qPCR after lentiviral knockdown or overexpression of HCAR. (C) Representative images of Alizarin red staining of BMSCs pellet culture during hypertrophic differentiation. Scale bar, 200 μm. (D) Quantitative PCR analysis of hypertrophic marker genes after hypertrophic induction. (E) Representative Western blot images of hypertrophic marker genes after hypertrophic induction. Data are shown as mean ± s.d. ∗ P < 0.05, ∗∗P < 0.01.