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. 2020 Aug 21;9(2):494–509. doi: 10.1016/j.gendis.2020.08.005

Figure 2.

Figure 2

Melatonin suppresses oncogene-induced SASP. (A) qPCR array analysis was used to assess the expression of SASP genes in HOSEpiCs subjected to retrovirus-mediated H-RasV12 expression with or without melatonin treatment for 8 days. The heatmap was generated according to RT-qPCR values that are arrayed from white (low value) to blue (high value). (B) The expression of SASP genes in HOSEpiCs that were transfected with a vector containing H-RasV12 or an empty control vector and treated with or without melatonin was determined through sequencing by RT-qPCR. (C, D) HOSEpiCs cultured with conditioned medium from either H-RasV12-mediated senescent HOSEpiCs (OIS-CM) or HOSEpiCs treated with the empty control vector (N-CM) and with or without melatonin for 8 days. (C) Representative SA-β-gal staining images and corresponding statistical data are shown. (D) The expression of SASP genes was measured using RT-qPCR. Statistical values are presented as the mean ± standard error of the mean of three independent experiments. A P < 0.05 was considered statistically significant using one-way analysis of variance. ∗, P < 0.05.