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. 2020 Aug 21;9(2):494–509. doi: 10.1016/j.gendis.2020.08.005

Figure 5.

Figure 5

Melatonin regulates the MAPK-NF-κB pathway activation. (A) Western blot analysis of RNA interference efficiency in HOSEpiCs transfected with scrambled or MAPK siRNAs. (B) Western blot analysis of the related proteins in NF-κB pathway activation in H-RasV12-induced senescent HOSEpiCs treated with or without melatonin using siRNA (siMAPK). HOSEpiCs were transfected with scramble siRNA as a control. (C) Immunofluorescence analysis of phosphorylated NF-κB (pNF-κB) in H-RasV12-induced senescent HOSEpiCs treated with or without melatonin and in the presence or absence of siMAPK. The nuclei were counterstained with DAPI (blue); scale bar, 50 μm. The results are presented as the mean ± standard deviation of three independent experiments. ∗∗, P < 0.01; ∗∗∗P < 0.001.