Melatonin modulates the extent of NF-κB-binding to SASP genes. (A) To demonstrate the binding of the indicated proteins to their target genes, a heatmap was generated according to ChIP-qPCR values. The ChIP-qPCR enrichments of no-antibody control (NA), NF-κB, H3K27ac, and H3 using primers spanning from −1 kb to +1 kb of the indicated SASP genes are arrayed from blue (no enrichment) to yellow (maximal enrichment). (B) The control H3 protein co-precipitated evenly with the ChIP targets. A heatmap was generated according to the ChIP-qPCR values of histone H3, which were between −1 kb and +1 kb of the indicated genes. (C) Representative ChIP-qPCR values of the IL-6 gene selected from the experiments described in (A). Statistical values are presented as the mean ± standard error of three independent experiments. A P < 0.05 was considered statistically significant using one-way analysis of variance. ∗, P < 0.05.