Supplementary figure 4.

Ccrl2 deficiency mice showed aggravated VAT inflammation manifested in accumulation of macrophages and increased M1/M2 ratio. (A) ccrl2 expression in VAT of ND 18w mice(n = 6) and ND 8W mice (AD n = 4 SVF n = 6). (B) ccrl2 expression in adipocyte and isolated macrophage from SVF of VAT(n = 3). (C) FCM analysis of proportion of macrophages in VAT or SAT of mice fed with ND of different genotypes(n = 6,8,8). (D) ATMs associated inflammation markers in SAT(n = 6 in per group). (E) serum IL-6 level in ND mice. (F) serum IL-6 level in HFD mice. Data are expressed as Mean ± SEM, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.