Fig. 5.

PD-1 blockade and CD27 deficiency on regulatory T cells synergizes to limit tumor growth. Irradiated RAG1 − / − mice were reconstituted with an equal mixture of bone marrow cells containing 50% FoxP3.LuciDTR-5 (CD45.1) cells and 50% cells from mice deficient for CD27. Eight weeks after reconstitution, mice were injected s.c. with MC38 tumor cells. Seven and nine days after tumor inoculation, mice were treated either with 30 ng/g DT i.v., with 250 µg a-PD-1 i.v. or with a combination of DT and a-PD-1. As for the control, some mice were left untreated. A Tumor growth was measured over time. B Cumulative survival curve of mice described in A. Data are representative of 3 independent experiments. Boxes and whiskers depict interquartile range and range of data (A, n = 14; B and C, n = 10). Statistical significance was determined with one-way ANOVA with Bonferroni’s multiple comparisons correction *p < 0.05