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3 different patient-derived brain tumor cell cultures (PDCs) were encapsulated in 3D hydrogels: aGBM (adult GBM), pGBM (pediatric GBM), and DIPG. An immortalized adult GBM cell line (U87) was also encapsulated as control. Hydrogel stiffness was modulated by tuning the PEG concentration (% w/v).
