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. 2021 Nov 10;13:64–81. doi: 10.1016/j.bioactmat.2021.11.002

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 3 — Evaluation of cellular responses of encapsulated preosteoblasts to the oxygen-generating scaffolds. a) Metabolic activity of preosteoblasts, which were 3D encapsulated in the oxygen-generating scaffolds, was determined using the Alamar Blue assay. The highest metabolic activity was obtained in the OGM₆₀ scaffolds and lowest one was found in the pristine GelMA group under hypoxia. b) Alkaline phosphatase (ALP) activity of the 3D encapsulated preosteoblasts was determined using an ALP assay. The OGM₆₀ scaffolds showed the highest ALP activity on day 14. (*p-value <0.05 was considered statistically insignificant).