Table 3.

Multi-lineage cell culture media.

Media	Composition
Osteogenic induction media	DMEM: F12
	10% FBS
	1% penicillin–streptomycin
	1% antibiotic–antimycotic
	10 nM dexamethasone
	10 mM β-glycerol phosphate
	50 µg/ml ascorbic-acid-2-phospahate
Osteogenic maintenance media	DMEM: F12
	10% FBS
	1% penicillin–streptomycin
	1% antibiotic–antimycotic
	10 nM dexamethasone
	5 mM β-glycerol phosphate
	50 µg/ml ascorbic-acid-2-phospahate
Adipogenic induction media	DMEM: F12
	10% FBS
	1% penicillin–streptomycin
	1% antibiotic–antimycotic
	10 µg/ml insulin
	1 µM dexamethasone
	500 µM 3-isobutyl-1-methylxanthine
Adipogenic maintenance media	DMEM: F12
	10% FBS
	1% penicillin–streptomycin
	1% antibiotic–antimycotic
	10 µg/ml insulin
Chondrogenic induction media	DMEM: F12
	10% FBS
	1% penicillin–streptomycin
	1% antibiotic–antimycotic
	10 nM dexamethasone
	50 µg/ml ascorbic-acid-2-phospahate
	10 ng/ml recombinant human TGF-β3
	40 μg/ml l-proline
	50 μg/ml ITS liquid media supplement