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. 2022 Feb 14;12:2465. doi: 10.1038/s41598-022-06520-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of reaction pH on the formation of isoprene and 2-methyl-3-butene-2-ol (2-MBO). Data are mean ± SE (n = 3). Two mM DMADP and 20 mM metal ions were reacted at 40 ℃ for 1 h at shown pH and the head space gas was analyzed by GC. For pH 5.5 and 6.5, used buffer was 45 mM 2-(N-morpholino) ethanesulfonic acid (MES) and for pH 7.5–10.5, 45 mM Tris–HCl was used. Formation rate of isoprene and 2-MBO was calculated by relative proportion of the peak area against that of standard isoprene gas.