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. 2022 Feb 14;12:2417. doi: 10.1038/s41598-022-06430-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunostaining of PD-L1. The cytoplasmic and nuclear PD-L1 were stained with antibody against PD-L1 (green). The nuclei was labeled with DAPI (blue). Dual immunofluorescence labeling of PD-L1 and DAPI in U87 cells treated to Cas9-g82, Cas9-g165, Cas9-g82/165, and Cas9-g82/165 + HDR.The untreated U87 cells served as control. Notable reduction of both cytoplasmic and nuclear PD-L1 was seen in U87 cells treated to Cas9-g82/165 + HDR.