Table 1.

Primer sequences used in this study.

Purpose	Primer name	Primer sequence
Cloning	Hu PD-L1 Cas9-g82 Forward	5Phos—CAC CGG GTT CCC AAG GAC CTA TAT G
Cloning	Hu PD-L1 Cas9-g82 Reverse	5Phos—AAA CCA TAT AGG TCC TTG GGA ACC C
Cloning	Hu PD-L1 Cas9-g165 Forward	5Phos—CAC CGT GGC TGC ACT AAT TGT CTA T
Cloning	Hu PD-L1 Cas9-g165 Reverse	5Phos—AAA CAT AGA CAA TTA GTG CAG CCA C
Transfection	Hu PD-L1 Repair Template	TGG CAT TTG CTG AAC GCA TTT ACT GTC ACG GTT CCC AAG GAC CTA TAA TAT TGA GAA ATG GAG GAT AAG AAC ATT ATT CAA TTT GTG CAT GGA G
Cloning	Ms PD-L1 Cas9-g70 Forward	5Phos—CAC CGG TTT ACT ATC ACG GCT CCA A
Cloning	Ms PD-L1 Cas9-g70 Reverse	5Phos—AAA CTT GGA GCC GTG ATA GTA AAC C
Cloning	Ms PD-L1 Cas9-g166 Forward	5Phos—CAC CGA GTA CAC CAC TAA CGC AAG C
Cloning	Ms PD-L1 Cas9-g166 Reverse	5Phos—AAA CGC TTG CGT TAG TGG TGT ACT C
Transfection	Ms PD-L1 Repair Template	TTC ACA GCC TGC TGT CAC TTG CTA CGG GCG TTT ACT ATC ACG GCT TAA GCG TTA GTG GTG TAC TGG GAA AAG GAA GAT GAG CAA GTG ATT C
T7E1	Hu PD-L1 Screen Forward	ACA TAA CCG ACC AGA TAA AGT GAT
T7E1	Hu PD-L1 Screen Reverse	AGT CTT CAA CAC TTG GAA TAT GTT T