FIGURE 2.

Hedgehog signaling pathway is downregulated in old and old regenerating hepatocytes. RNAseq was performed in hepatocytes isolated from 0 and 48 h post‐PH in young and old mice (n = 3 mice/group/time). (a) Left, heatmap displaying log2 fold change of signficantly altered Hedgehog pathway genes in old, young regenerating and old regenerating hepatocytes normalized to young hepatocytes. Right, gene set enrichment analysis (GSEA) of Hedgehog signaling hallmark signature in old (top) and old regenerating (bottom) hepatocytes. (b) Left, graph displaying normalized enrichment score (NES) of signicantly downregulated Hedgehog target pathways in old (top) and old regenerating (bottom) hepatocytes normalized to young hepatocytes. Right, gene set enrichment analysis (GSEA) of Hedgehog target pathways: Wnt and NOTCH signatures in old (top) and old regenerating hepatocytes (bottom)