FIGURE 2.

CultureAGE phenotype is independent of cellular senescence phenotype and requires replicative expansion. Boxplot displaying varying CultureAGE scores in young (untreated and DMSO, passage 1 or 2), passage‐independent (passage 2) senescence induction doxorubicin (1 µM), etoposide (12.5 µM), irradiation (10 gy), old (passage 3–5.5), and LTK1 immortalized cells (passage 5). Passaged label denotes cells were mitotically expanded, where β‐gal label establishes a binary senescence cutoff based on flow cytometry data in Figure S3. Statistical significance calculations were determined via one‐way ANOVA and multiple group comparisons