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89Zr-labeling of anti-PD-L1. (A) Diagram of 89Zr-anti-PD-L1 (left), nonreduced sodium dodecyl sulfate PAGE (middle), and MALDI time-of-flight results (right). (B) Radioactivity profile of PD-10 column–eluted fractions (left), autoradiography on native PAGE (middle), and in vitro stability (right). DFO = deferoxamine; FBS = fetal bovine serum; Mal = maleimide; PBS = phosphate-buffered saline; SH = sulfohydryl; TCEP = tris(2-carboxyethyl)phosphine
