FIGURE 4.

Effects of chemotherapeutic agents on CT26 cells. (A) Stimulatory effects of 24-h treatment with graded doses of cisplatin (CDDP) alone (left), CDDP plus 5-fluorouracil (middle), or olaparib (right) on ⁸⁹Zr-anti-PD-L1 binding. (B) PD-L1 immunoblots and β-actin–corrected band (left), flow cytometry of PD-L1–positive cells (middle), and ⁸⁹Zr-anti-PD-L1 binding (right). Bars are means ± SDs. Binding data are from triplicate samples per group. *P < 0.05, compared with controls. **P < 0.01, compared with controls. ^† P < 0.005, compared with controls.