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AKT and PTEN signaling in gemcitabine effect on CT26 cells. (A) Immunoblots and quantified protein band intensities (corrected by appropriate controls) for PD-L1, p-AKT, and p-PTEN. (B) Effects of rapamycin (1 μM) and MHY1485 (2 μM) on immunoblots and band intensities for p-mTOR (corrected by t-mTOR) and PD-L1 (corrected by β-actin). Bars are means ± SDs. *P < 0.05. † P < 0.005. ‡ P < 0.001.
