Fig. 1. Kidney functions and inflammation of mice in Sham, CLP, CLP + DMSO and CLP + ISO-1 group were assessed through observation of pathological sections, serum creatinine, urea nitrogen, MIF and IL-1β levels.

A H&E stained pathological sections. Normal glomerulus (blue arrow), normal kidney tubules (green arrow), injured glomerulus (yellow arrow), aggregation of Inflammatory cell (gray arrow), disappear of brushlike margin and vacuolation of renal tubular cells (black arrow). B–E Creatinine, urea nitrogen, MIF and IL-1β levels in serum. Scale bar = 50 μm. F, G Morphology of renal tubular epithelial cell in Sham group and in CLP group was observed under TEM (magnification, 2000x). H, I Orangelles and nucleus of healthy cells in Sham group and in CLP group (magnification, 10,000x). Nucleus (blue arrow), mitochondria (orange arrow), glycogen granules (yellow arrow), endoplasmic reticulum (green arrow), intact cell membrane (pink arrow), broken cell membrane (red arrow), vesicles (purple arrow). Data was mean ± SD from five mice. Each point represented an independent data. Group comparisons were performed by one-way ANOVA followed by Tukey’s post hoc test. (N = 5/group, ^#P < 0.05 vs Sham group, *P < 0.05 and **P < 0.01 vs CLP + DMSO group).