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. 2022 Feb 14;8:61. doi: 10.1038/s41420-022-00859-z

Fig. 6. the mechanism of MIF promoting pyroptosis was verified via JSH-23 inhibiting p65 transfer to the nucleus.

Fig. 6

A MIF and NLRP3 expressions relative to GAPDH were measured by western blot. B HK-2 cells of +LPS and +JSH-23+LPS group were stained by annexin-V-FITC and PI. C PI stained cells were counted by flow cytometry. D Cell viability of HK-2 cells was evaluated via CCK-8 method. Data were mean ± SD from three independent experiments. Each point represented an independent data. Group comparisons were performed by (A) one-way ANOVA followed by Tukey’s post hoc test, (C) unpaired T-test and (D) two-way ANOVA followed by Tukey’s post hoc test. (N = 3/group, #P < 0.05 vs +PBS group, *P < 0.05 vs +LPS group, NS means no significant difference).