Figure 2.

Components for the cryofixation of capillaries

(A) Empty puck and a storage box inside the freezing apparatus before addition of liquid nitrogen.

(B) Top: Puck filled with capillaries (blue) and pins (orange) for fixed orientation of a puck in a storage box filled with liquid nitrogen. Bottom: Propane well (at 20°C–24°C temperature) in which the capillary is plunged positioned on the propane heater to avoid overcooling of propane.

(C) The grabber firmly holding a capillary before plunging down.