Figure 7.

Segmentation of cellular organelles using Amira

On the left: segmentation editor panel including materials and tools. On the right: virtual slice through the reconstructed SXT volume with manually segmented nucleus (blue) and based on the absorption threshold (0.28 - 0.45 μm-1) semi-automatically segmented mitochondria (red). The insert shows expected outcome of segmentation procedure, i.e., 3D rendering of the cell, showing heterochromatin (blue) and euchromatin (magenta) defining the nucleus, mitochondria (magenta), lipid droplets (yellow), ER (cyan), and plasma membrane (grey).