REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
SARS-CoV-2 (isolate Bavpat1/2020)	European Virology Archive	Ref-SKU: 026V-03883
Chemicals, peptides, and recombinant proteins
PBS	Thermo Scientific	Cat.#10010023
4000 Series monosized microbeads in polystyrene	Thermo Scientific	Cat.#4206A (6 μm) Cat.#4208A (8 μm) Cat.#4210A (10 μm)
Cell Strainer	pluriSelect	SKU 43-10040-40 (40 μm) SKU 43-10020-40 (20 μm)
Paraformaldehyde (optional, for chemical fixation)	Thermo Scientific	Cat.#J61899-AP
Formaldehyde (optional, for chemical fixation)	Thermo Scientific	Cat.#28906
Glutaraldehyde EM grade	Electron Microscopy Sciences	Cat. #E16210
Experimental models: Cell lines
Calu-3	ATCC	Cat.#HTB-55
A549-ACE2	(Klein et al., 2020)	N/A
HEK293T-ACE2	Gift by Prof. Becker	N/A
Human B lymphocytes	Coriell Institute of Medical Research	GM12878
Software and algorithms
ImageJ – Fiji 1.53 c	(Schneider et al., 2012)	https://imagej.nih.gov/ij/
GraphPad Prism 9	GraphPad	https://www.graphpad.com/scientific-software/prism/
Amira 2019.1	Thermo Scientific	https://thermofisher.com/amira-avizo
IMOD – 4.10.42	(Kremer et al., 1996)	https://bio3d.colorado.edu/imod/
AREC reconstruction	(Parkinson et al., 2012)	N/A