Fig. 3.

Anti-inflammation of naringin in vitro and in vivo. A–D Effect of naringin on LPS-induced mRNA expression of COX-2, iNOS, IL-1β, and IL-6 in RAW 264.7 macrophages. The concentration of LPS is 1 μg/mL. A COX-2 mRNA relative expression level; B IL-1β mRNA relative expression level; C iNOS mRNA relative expression level; D IL-6 mRNA relative expression level. Different letters show significant differences (p < 0.05). E–G Anti-inflammation effect of naringin on myocardial ischemia/reperfusion injury in rats. E Representative images of the Western blot results. F HMGB1 protein Expression level; G p-P38 protein expression levels. *p < 0.05 vs. the Sham group; ^#p < 0.05 vs. the I/R group. COX-2, cyclooxygenase-2; HMGB1, high mobility group box 1 protein; IL-1β, interleukin-1β; IL-6, interleukin 6; iNOS, inducible nitric oxide synthase; I/R, ischemia/reperfusion; LPS, lipopolysaccharide; Nar, naringin