Fig. 4. Multidimensional scaling plots based on the transcriptomes of 21 clinical lasR* and 28 lasR^WTP. aeruginosa isolates under planktonic and biofilm growth conditions.

For transcriptional profiling, bacteria were grown in rich LB media until an OD₆₀₀ of 2 at 37 °C and 180 rpm for planktonic growth (a), or statically in biofilms for 48 h (b). Reads were normalized for the individual library size and genes with coverage below 1 count per million were excluded from the analysis. Ellipses display the 95% confidence intervals.