Fig. 7. Expression of genes that are affected by the presence of lasR in the clinical lasR* isolates in PA14 and its isogenic ∆lasR, ∆rhlR, and ∆phoB single and double mutants.

a Gene expression values (log₁₀(nrpg + 1)) of the lasR core regulon genes (n = 136) and b of the lasR repressed (n = 412) genes in the ∆lasR, ∆rhlR, ∆phoB, ∆phoB/∆lasR, and ∆lasR/∆rhlR deletion mutants under planktonic (left) and biofilm (right) growth conditions. Asterisk: p-value < 0.05, Wilcoxon rank-sum test. Boxplot elements are: center line—median; box limits—upper and lower quartiles; whiskers—1.5x interquartile range; points—gene expression values (log₁₀(nrpg + 1)).