Table 3.
Strains and plasmids used in this study.
| Strain | Genotype/description | Reference |
|---|---|---|
| E. coli | ||
| DH5α | F-, supE44, ΔlacU169, (ϕ80 lacZDM15), hsdR17, (rk-mk+), recA1, endA1, thi1, gyrA, relA | 61 |
| P. aeruginosa | ||
| PA14 WT | Wild type strain | 24 |
| PA14 ∆lasR | PA14 derivative with a full deletion of lasR | 36 |
| PA14 ∆rhlR | PA14 derivative with a full deletion of rhlR | This study |
| PA14 ∆lasR ∆rhlR | PA14 derivative carrying a double lasR/rhlR deletion | This study |
| PA14 ∆phoB | PA14 derivative with a full deletion of phoB | This study |
| PA14 ∆lasR ∆phoB | PA14 derivative carrying a double lasR/phoB deletion | This study |
| Plasmids | ||
| pS448•CsR | SmR, oriV pRO1600/ColE1, oriT, xylS, Pm→cas9, PEM7→sgRNA | 48 |
| pSEVA658-ssr | GmR, oriV RSF1010, oriT, xylS, Pm→ssr | 62 |
| pSEVA624 | GmR, oriV RK2, oriT, lacIq/Ptrc expression cassette | 63 |
| pSH624-ssr | GmR, pSEVA624 cloned with the ssr recombinase sequence | This study |
| pS448•CsR-rhlR_spc2 | SmR, derivative of pS448•CsR carrying a PEM7→rhlR-targeting sgRNA | This study |
| pS448•CsR-phoB_spc2 | SmR, derivative of pS448•CsR carrying a PEM7→phoB-targeting sgRNA | This study |