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. 2021 Dec 26;9(5):2103543. doi: 10.1002/advs.202103543

Figure 1.

Figure 1

Increased infiltration of neutrophils in GC is correlated with disease stage and poor survival in patients. A,C) Neutrophil percentage in CD45+ cells or the total number of neutrophils per million total cells among TNM stages (I+II vs III+IV) in each tissue of patients with GC by gating on CD45+CD11b+CD66b+CD15+ cells or counting. Cumulative results from 51 GC patients and 36 healthy donors are shown. B) Dot plots of surface molecule staining for neutrophils gating on CD45+CD11b+ cells. D) Representative analysis of CD15+ (brown) neutrophil distributions in tissues of GC patients by immunohistochemical staining. Scale bars: 100 µm. E) Neutrophil number among TNM stages (I+II vs III+IV) in each tissue of patients with GC by immunohistochemical staining and counting. Cumulative results from 125 GC patients were shown. F) Neutrophil number among TNM stages was compared. G) Kaplan‐Meier plots for overall survival and disease‐free survival by median neutrophil number (132 per field). Data are mean ± SEM and analyzed by Student's t‐test, Mann‐Whitney U‐test, and one‐way ANOVA. *P < 0.05, **P < 0.01, n.s. P > 0.05 for groups connected by horizontal lines. Neu (NO), neutrophil number.