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. 2021 Dec 26;9(5):2103543. doi: 10.1002/advs.202103543

Figure 6.

Figure 6

Tumor‐infiltrating and tumor‐conditioned neutrophils suppress CD8+ T‐cell immunity through FasL and PD‐L2. A) Representative image of CD15+ neutrophil (green) and CD8+ T cell (red) interactions in tumor tissues of GC patients by immunofluorescence. Scale bars: 20 µm. B) The correlations between neutrophils and CD8+ T cells in human GC tumors were analyzed. C) CFSE‐labeled peripheral CD8+ T cells of GC patients were co‐cultured for 5 d with autologous neutrophils from non‐tumor or tumor tissues with or without anti‐FasL and/or anti‐PD‐L2 antibody. Representative data and statistical analysis of T cell proliferation and proliferated IFN‐γ‐producing T cells were shown (n = 3). D) CFSE‐labeled peripheral CD8+ T cells of donors were co‐cultured for 5 d with autologous NTCS‐conditioned neutrophils or TTCS‐conditioned neutrophils with or without anti‐FasL and/or anti‐PD‐L2 antibody. Representative data and statistical analysis of T cell proliferation and proliferated IFN‐γ‐producing T cells were shown (n = 3). E) CFSE‐labeled tumor‐specific CD8+ T cells of donors were co‐cultured for 5 d with autologous normal neutrophils or TTCS‐conditioned neutrophils with or without anti‐FasL and/or anti‐PD‐L2 antibody. Representative data and statistical analysis of T cell proliferation and proliferated IFN‐γ‐producing T cells were shown (n = 3). Data are mean ± SEM and analyzed by Student's t‐test, Mann‐Whitney U‐test, and one‐way ANOVA. *P < 0.05, **P < 0.01, n.s. P > 0.05 for groups connected by horizontal lines.